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pegfp n1 vector  (Addgene inc)


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    Structured Review

    Addgene inc pegfp n1 vector
    Pegfp N1 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pegfp+n1+vector/pcDNA3%2E1+puro+Nodamura+B2+(Plasmid+%2317228)/pmc12628023-87-13-16
    Average 93 stars, based on 18 article reviews
    pegfp n1 vector - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Amplification:

    Article Title: Mutation of CRYAB encoding a conserved mitochondrial chaperone and antiapoptotic protein causes hereditary optic atrophy
    Article Snippet: .. The full-length human CRYAB cDNAs were amplified with primers — 5′- CCGCTCGAGATGGACATCGCCAT -3′ (forward) and 5′- CGACCGGTGGGTATTTCTTGGGG -3′ (reverse) — and cloned into the XhoI and AgeI restriction sites of pEGFP-N1 vector (Addgene plasmid 172281). ..

    Clone Assay:

    Article Title: Mutation of CRYAB encoding a conserved mitochondrial chaperone and antiapoptotic protein causes hereditary optic atrophy
    Article Snippet: .. The full-length human CRYAB cDNAs were amplified with primers — 5′- CCGCTCGAGATGGACATCGCCAT -3′ (forward) and 5′- CGACCGGTGGGTATTTCTTGGGG -3′ (reverse) — and cloned into the XhoI and AgeI restriction sites of pEGFP-N1 vector (Addgene plasmid 172281). ..

    Article Title: Artesunate induces ferroptosis in gastric cancer by targeting the TFRC-HSPA9 axis for iron homeostasis regulation
    Article Snippet: .. To construct vectors for fluorescent protein expression, TFRC cDNA was inserted into the pEGFP-N1 vector (#172281, Addgene), while HSPA9 cDNA was cloned into the pSicoR-mCherry vector (#21907, Addgene). ..

    Plasmid Preparation:

    Article Title: Mutation of CRYAB encoding a conserved mitochondrial chaperone and antiapoptotic protein causes hereditary optic atrophy
    Article Snippet: .. The full-length human CRYAB cDNAs were amplified with primers — 5′- CCGCTCGAGATGGACATCGCCAT -3′ (forward) and 5′- CGACCGGTGGGTATTTCTTGGGG -3′ (reverse) — and cloned into the XhoI and AgeI restriction sites of pEGFP-N1 vector (Addgene plasmid 172281). ..

    Article Title: Structural insights into histone binding and nucleosome assembly by chromatin assembly factor-1
    Article Snippet: .. Briefly, tandem copies of the 147-bp W601 DNA fragment were inserted into a pEGFP-N1 vector (Addgene), and recombinant plasmids were produced in large bacterial culture. ..

    Article Title: Aquaporins enriched in endothelial vacuole membrane regulate the diameters of microvasculature in hyperglycaemia.
    Article Snippet: . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Aquaporins enriched in endothelial vacuole membrane regulate the diameters of microvasculature in hyperglycaemia

    Article Title: Artesunate induces ferroptosis in gastric cancer by targeting the TFRC-HSPA9 axis for iron homeostasis regulation
    Article Snippet: .. To construct vectors for fluorescent protein expression, TFRC cDNA was inserted into the pEGFP-N1 vector (#172281, Addgene), while HSPA9 cDNA was cloned into the pSicoR-mCherry vector (#21907, Addgene). ..

    Article Title: Mechanism for controlled assembly of transcriptional condensates by Aire
    Article Snippet: .. For mGFP-p300 variant fusions, p300 variants were subcloned into a modified pEGFP-N1 vector where A206K point mutation was introduced in enhanced GFP to encode monomeric GFP. pET22b-HisGb1-STAT1(aa 710- 750) + CBP TAZ2 (aa 1764-1855) was a gift from Peter Wright (Addgene plasmid # 99342)7. mouse Aire CTT (aa 480-552) and mouse Aire PHD1 (aa 295-349) were subcloned into pET47b. .. DNA encoding CHD4 PHD2 (aa 439-493) was synthesized by Integrated DNA Technologies (IDT); this cDNA was used as a template for subcloning into pET47b. cDNA for Sp110 CARD (aa 6-110) was amplified from MegaMan Human Transcriptome Library (Stratagene).

    Recombinant:

    Article Title: Structural insights into histone binding and nucleosome assembly by chromatin assembly factor-1
    Article Snippet: .. Briefly, tandem copies of the 147-bp W601 DNA fragment were inserted into a pEGFP-N1 vector (Addgene), and recombinant plasmids were produced in large bacterial culture. ..

    Produced:

    Article Title: Structural insights into histone binding and nucleosome assembly by chromatin assembly factor-1
    Article Snippet: .. Briefly, tandem copies of the 147-bp W601 DNA fragment were inserted into a pEGFP-N1 vector (Addgene), and recombinant plasmids were produced in large bacterial culture. ..

    Cell Culture:

    Article Title: EF24, a Curcumin Analog, Reverses Interleukin-18-Induced miR-30a or miR-342-Dependent TRAF3IP2 Expression, RECK Suppression, and the Proinflammatory Phenotype of Human Aortic Smooth Muscle Cells
    Article Snippet: ASMCs were transfected with 80 nM of mimics or NC using the Neon ® Transfection System (MPK-5000; Invitrogen/Thermo Fisher Scientific, Waltham, MA, USA) using the following parameters: pulse voltage—1300 V; pulse width—20 ms; pulse number—2; the tip type—10 μL. .. They were then cultured for 24 h. The transfection efficiency of ASMC was ∼59% with 2% cell death, as determined using the pEGFP-N1 vector (#6081-5; Addgene, Watertown, MA, USA). ..

    Article Title: EF24, a Curcumin Analog, Reverses Interleukin-18-Induced miR-30a or miR-342-Dependent TRAF3IP2 Expression, RECK Suppression, and the Proinflammatory Phenotype of Human Aortic Smooth Muscle Cells.
    Article Snippet: ASMCs were transfected with 80 nM of mimics or NC using the Neon® Transfection System (MPK-5000; Invitrogen/Thermo Fisher Scientific, Waltham, MA, USA) using the following parameters: pulse voltage—1300 V; pulse width—20 ms; pulse number—2; the tip type—10μL. .. They were then cultured for 24 h. The transfection efficiency of ASMC was ∼59% with 2% cell death, as determined using the pEGFP-N1 vector (#6081-5; Addgene, Watertown, MA, USA). ..

    Transfection:

    Article Title: EF24, a Curcumin Analog, Reverses Interleukin-18-Induced miR-30a or miR-342-Dependent TRAF3IP2 Expression, RECK Suppression, and the Proinflammatory Phenotype of Human Aortic Smooth Muscle Cells
    Article Snippet: ASMCs were transfected with 80 nM of mimics or NC using the Neon ® Transfection System (MPK-5000; Invitrogen/Thermo Fisher Scientific, Waltham, MA, USA) using the following parameters: pulse voltage—1300 V; pulse width—20 ms; pulse number—2; the tip type—10 μL. .. They were then cultured for 24 h. The transfection efficiency of ASMC was ∼59% with 2% cell death, as determined using the pEGFP-N1 vector (#6081-5; Addgene, Watertown, MA, USA). ..

    Article Title: EF24, a Curcumin Analog, Reverses Interleukin-18-Induced miR-30a or miR-342-Dependent TRAF3IP2 Expression, RECK Suppression, and the Proinflammatory Phenotype of Human Aortic Smooth Muscle Cells.
    Article Snippet: ASMCs were transfected with 80 nM of mimics or NC using the Neon® Transfection System (MPK-5000; Invitrogen/Thermo Fisher Scientific, Waltham, MA, USA) using the following parameters: pulse voltage—1300 V; pulse width—20 ms; pulse number—2; the tip type—10μL. .. They were then cultured for 24 h. The transfection efficiency of ASMC was ∼59% with 2% cell death, as determined using the pEGFP-N1 vector (#6081-5; Addgene, Watertown, MA, USA). ..

    Construct:

    Article Title: Artesunate induces ferroptosis in gastric cancer by targeting the TFRC-HSPA9 axis for iron homeostasis regulation
    Article Snippet: .. To construct vectors for fluorescent protein expression, TFRC cDNA was inserted into the pEGFP-N1 vector (#172281, Addgene), while HSPA9 cDNA was cloned into the pSicoR-mCherry vector (#21907, Addgene). ..

    Expressing:

    Article Title: Artesunate induces ferroptosis in gastric cancer by targeting the TFRC-HSPA9 axis for iron homeostasis regulation
    Article Snippet: .. To construct vectors for fluorescent protein expression, TFRC cDNA was inserted into the pEGFP-N1 vector (#172281, Addgene), while HSPA9 cDNA was cloned into the pSicoR-mCherry vector (#21907, Addgene). ..

    Variant Assay:

    Article Title: Mechanism for controlled assembly of transcriptional condensates by Aire
    Article Snippet: .. For mGFP-p300 variant fusions, p300 variants were subcloned into a modified pEGFP-N1 vector where A206K point mutation was introduced in enhanced GFP to encode monomeric GFP. pET22b-HisGb1-STAT1(aa 710- 750) + CBP TAZ2 (aa 1764-1855) was a gift from Peter Wright (Addgene plasmid # 99342)7. mouse Aire CTT (aa 480-552) and mouse Aire PHD1 (aa 295-349) were subcloned into pET47b. .. DNA encoding CHD4 PHD2 (aa 439-493) was synthesized by Integrated DNA Technologies (IDT); this cDNA was used as a template for subcloning into pET47b. cDNA for Sp110 CARD (aa 6-110) was amplified from MegaMan Human Transcriptome Library (Stratagene).

    Modification:

    Article Title: Mechanism for controlled assembly of transcriptional condensates by Aire
    Article Snippet: .. For mGFP-p300 variant fusions, p300 variants were subcloned into a modified pEGFP-N1 vector where A206K point mutation was introduced in enhanced GFP to encode monomeric GFP. pET22b-HisGb1-STAT1(aa 710- 750) + CBP TAZ2 (aa 1764-1855) was a gift from Peter Wright (Addgene plasmid # 99342)7. mouse Aire CTT (aa 480-552) and mouse Aire PHD1 (aa 295-349) were subcloned into pET47b. .. DNA encoding CHD4 PHD2 (aa 439-493) was synthesized by Integrated DNA Technologies (IDT); this cDNA was used as a template for subcloning into pET47b. cDNA for Sp110 CARD (aa 6-110) was amplified from MegaMan Human Transcriptome Library (Stratagene).

    Mutagenesis:

    Article Title: Mechanism for controlled assembly of transcriptional condensates by Aire
    Article Snippet: .. For mGFP-p300 variant fusions, p300 variants were subcloned into a modified pEGFP-N1 vector where A206K point mutation was introduced in enhanced GFP to encode monomeric GFP. pET22b-HisGb1-STAT1(aa 710- 750) + CBP TAZ2 (aa 1764-1855) was a gift from Peter Wright (Addgene plasmid # 99342)7. mouse Aire CTT (aa 480-552) and mouse Aire PHD1 (aa 295-349) were subcloned into pET47b. .. DNA encoding CHD4 PHD2 (aa 439-493) was synthesized by Integrated DNA Technologies (IDT); this cDNA was used as a template for subcloning into pET47b. cDNA for Sp110 CARD (aa 6-110) was amplified from MegaMan Human Transcriptome Library (Stratagene).



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